The research purpose has been done to produce egg yolk freeze dry and egg yolk
nanoparticles that quality in tris diluents to frozen semen storage to support germ plasm
conservation of Garut ram. Specifically the objectives of the study can be formulated as
follows: 1) Produce egg yolk freeze dry product through freeze drying process, 2) Minimize
the size of the egg yolk through the nano-milling process, 3) Prove the potential of egg yolk
freeze dry and egg yolk nanoparticles in tris diluents to maintain the quality of sperm
resulting from storage of semen. The result of the research indicate fresh egg yolk, freeze
dry egg yolk, commercial egg yolk, and milling egg yolk 110 have size particle and
polydispersity index each of those are 13920.00±1065.55; 1.00±0.00, 1296.00±86.73;
0.70±0.04, 877.90±168.86; 0.34±0.04, and 403.06±7.05; 0.46±0.03. Egg yolk milling 110
with particle size (403.06±7.05 nm) has the smallest size when compared to fresh egg yolk,
freeze dery egg yolk, adn commercial egg yolk. The potential zeta analysis of milling egg
yolk 110 have good stability with zeta potential value is -16.68 ± 0.37 mV. The Zeta
potensial test is only done on milling egg yolk 110. Morphology of freeze dry egg yolk and
milling egg yolk 110 are almost the same, while commercial egg yolk is different. The
conclusion of the research indicate milling egg yolk 110 has a smaller size compared to fresh
egg yolk, freeze dry egg yolk, and commercial egg yolk. Milling egg yolk has good
uniformity and good particle stability.